This study was devised to observe the inhibitory effect of growth rate of human colon cancer cells. by Eucommial leaf extract, in vitro.
Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were used for the experiment.
Each extract of Ecommial leaf was prepared by extraction with water, 95% alcohol, acetone, chloroform and petroleum ether, and then the inhibitory effect of each extract on the growth rate of cells was compared with control group and each other.
The expermental results obtained are summarized as follows;
1. Inhibitory effects on growth rate of human colon cancer cells were strongest in the petroleum. ether extract and next in the chloroform extract.
2. Inhibitory effects on the growth rate of the cancer cells by extracts of water, 95% alcohol and acetone were weaker than that of petroleum ether and chloroform.
3. Inhibitory effect of each extract on the cancer cell growth was shown most strong activity in HT-29, and was in order of HRT-18 and HCT-48.
In view of the results, it could be suggested that inhibitory effects of non-polar solvent´s extracts against the cancer cell growth were more stronger than that of polar solvents and the effects were indicated defference according to the species of the cells.
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